Essay about The Use of Recombinant DNA Technology - 844 Words

The Use of Recombinant DNA Technology Recombinant DNA technology is the technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism.(1) This is the process of using recombinant DNA technology to enable the rapid production of human protein from a single gene of insulin. Firstly the single gene required must be isolated. This can be done three ways: Either by working backwards from the protein- Finding the amino acid sequence for the protein needed, the order of bases can be established using known genetic code. New DNA can be made from this sequence of bases resulting in artificial gene made from complementary DNA. By†¦show more content†¦They are short circular strands of DNA found in some bacteria. The joining process is controlled by the enzyme ligase and is called ligation. The new circular DNA molecule produced is called recombinant DNA as it contains genes from the original plasmid and the human gene. Finally if to discover if the uptake of DNA had been successful a gene for antibiotic resistance is used. If bacterial cells have successfully taken up the recombinant DNA then it will be resistant to the antibiotic. They are then grown in fermentors. Cells that have not taken up the DNA will be killed by the antibiotic. (3) Recombinant DNA technology has many different uses. It is used for genetically engineered micro organisms. This is when human genes are inserted into bacteria which are then grown in fermentors. The bacteria then produce a human protein. So, large amounts of insulin and human growth hormone can be produced cheaply. It is used to map human chromosomes (The human genome project). The aim of this is to create a map of all the human chromosomes identifying the precise location of every gene. This will help to develop a new gene therapy treatment It can be use for identification of mutations-people may be tested for the presence of mutated proteins that may be associated with breast cancer, retinoblastoma, and neurofibromatosis. It can be used forShow MoreRelatedThe Debated Applications of Recombinant DNA Technology Essay703 Words   |  3 Pagesâ€Å"[...] any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use.† This statement is often used to describe the fundamental aim of biotechnology around the world. Karl Ereky, one of the foremost proponents of the term biotechnology foresaw that â€Å"merging biology and technology could be used to transform living substances into products that are more useful than in their natural state,† thus benefitingRead MoreThe Potential Of Manipulating Genetic Engineering1571 Words   |  7 Pagesproducts to consume. With two conflicting stances on this DNA technology, there essentially cannot be a resolution to this controversy; only arguments and reasons. However, what exactly is each side’s rationale? Through observation and analyzation, each stance’s reasonings will appear; as well as vital information about this DNA Technology itself. Also, this will allow me to confirm my beliefs on this controversial topic. As of right now, the technology appears to effectively split the human society, inRead MoreThe Use Of Recombinant Dna For Science And Medicine909 Words   |  4 PagesThe use of recombinant DNA technology has been a huge advancement to science. In the early 1970s the first recombinant DNA was produced (â€Å"1972 First†, 2013). Researchers Stanley Cohen and Hebert Boyer used restriction enzymes to cut DNA at specific sites, and then fused them back together to form their recombinant vector (â€Å"1972 First†, 2013; Cederbaum, S., et al., 1984). 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Old ) DNA can be cut using the enzyme Restriction endonuclease, which recognisesRead MoreEssay on Facts of Cloning1032 Words   |   5 Pagescloning. This may be the most popular type of cloning known in society, but there are two others used in the scientific community. The two types are, recombinant DNA cloning (DNA cloning), and therapeutic cloning. The type of cloning used for Dolly was reproductive cloning, which is, a technology used to generate an animal that has the same nuclear DNA as another animal that is currently in existence or once was. More specifically there is a process known as SCNT (somatic cell nuclear transfer). Read MoreThe Benefits of Genetic Engineering Essay1459 Words   |  6 PagesThere are many aspects of genetic engineering and to thoroughly understand it looking into each is absolutely necessary. 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With the addition of recombinant DNA technology scientists have been able to manufacture human insulin, the hepatitis B vaccine, the calf enzyme used in cheese- making, biodegradableRead MoreDna Lab Report1345 Words   |  6 Pages The genomic DNA sample concentration of 28.5 ng/ÃŽ ¼L and its A260/A280 ratio of 1.85 indicates that the DNA was relatively pure since a 260/280 ratio of ~1.8 is generally accepted as pure for DNA (Cox, Doudna O’Donnell, 2015). The ratio of white colonies to blue colonies observed on the blue-white screen plate was 31:37, which shows that there was a greater number of E. coli DH5-ÃŽ ± that did not contain the plasmid with the foreign gene insert. Therefore, out of all of the E. coli DH5-ÃŽ ± cells that